TNFRSF1B
Reactivity: Mouse
WB, FACS, IP, Func, IA
Host: Rat
Monoclonal
HM102
Biotin
Application Notes
ELISA: To detect hsTNF-receptor II by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II. To detect hsTNF-receptor II by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with a biotinylated Anti-Human sTNF-receptor II (PP1222B) as adetection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinanthsTNF-receptor II. Western Blot: This antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction withcompatible secondary reagents the detection limit for recombinant hsTNF-receptor II is1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
Target
TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor